The Unknown Bacteria Plate Chosen - 926 Words

Unknown Bacteria I. Introduction The unknown bacteria plate chosen was plate #2. It was identified to be Micrococcus luteus. It is a gram positive, Coccus bacteria that is commonly found in dust, water, soil, and the air. M. luteus also thrives in the human mouth and upper respiratory tract. Sir Alexander Fleming discovered it in 1928 before he identified penicillin. It is part of the normal flora on human skin as well as other mammals. Since it is part of normal flora it is normally not pathogenic, but can become opportunistic in an immune-deficient person. It has been known to cause septic shock, UTI’s, and even pneumonia. Micrococcus luteus is both urease and catalase positive. It does not utilize tryptophan for indole production. It is a facultative anaerobe. Mobility is not present for this bacterium. Starch is also not hydrolyzed and oxidase is not present. II. Materials and Methods The Gram stain was used to determine if the bacteria was gram positive or negative. A negative test shows a pink color and a positive test is a purple color. When a bacterium is negative it is because it has an outer membrane and a thin layer of peptidoglycan that is much harder to stain. A positive bacterium has a thick layer of peptidoglycan and no outer membrane that can be penetrated by crystal violet. To perform this test, a small drop of water is placed on a clean microscope slide. A metal loop that has been properly sterilized in the blue flame and allowed time to cool is used toShow MoreRelatedIdentifying And Identify Two Unknown Bacteria From A Mixed Culture1737 Words   |  7 Pagesand identify two unknown bacteria from a mixed culture. The ability to properly evaluate biochemical test results is also necessary for the identification to be successful. The goal was to apply all of the methods and techniques that have been learned in the microbiology laboratory course for the proper identification of unknown bacteria. A certain amount of bacteria that were used throughout the course were possible bacteria that could be found in a mixed culture. The bacteria that were identifiedRead MoreThe Laboratory Without Contamination Is Essential For The Study And Classifications Of The Unknown Bacterial Culture1203 Words   |  5 Pagesinoculating. The organism chosen in class will be used to construct further testing. The purpose of this lab experiment was to find the identity of the unknown bacterial culture and use all the methods and the assessments that were introduced in the class. By doing that we had to use different techniques that guide us to find the right bacterium. A flowchart was given for the unknown to help us find the right bacterium that was in the test tube. 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Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics. The differential tests used to identify the unknown cultures were Gram staining, oxidase, indole test, urea test, and casein test. MATERIALS AND METHODS: The unknown bacteria were given out by the